show Abstracthide AbstractBurkholderia spp. are often resistant to antibiotics, and infections with these organisms are diffi-cult to treat. A potential alternative treatment for Burkholderia spp. infections is bacteriophage (phage) therapy; however, it can be difficult to locate phages that target these bacteria. We sequenced and compared the genomes of Burkholderia spp. clinical isolates and prophage-derived phages that were isolated from them.